Inhibitor Screening: The Static Flask Test
By: Michelle Elliott 10/8/03
Updated 06/27/08 by Adelon Agustin

Screening of inhibitors is done to determine the effectiveness and efficiency of experimental inhibitors under aerobic and anaerobic atmospheres. Inhibitor testing can be done using two different methods: Static Flask Test or use of the CSTR. When using the CSTR, consult the operator’s manual. This procedure explanation only covers the Static Flask Test.

This test is based on a small scale and usually the first test done on an experimental inhibitor. It will provide an answer to whether or not further testing is needed.

Equipment:

|2 three-necked round bottom flask (50ml) |2 Mass flow meter for nitrogen |
|2 mini magnetic stir bars, placed in the flasks |Heating Mantle |
|6 rubber septa, to fit the round bottom flasks |Stirring Mantle, with large stir bar |
|2 Syringe Needles, 20 gauge, 6 in long, luer hub and deflecting |Temperature controller, with thermocouple |
|tip |Glycerol |
|2 needles, 21gauge, 1in long, used to vent the flasks |Metal pan |
|2 glass syringes, 5cc with 18 gauge, 4 in detachable needles |Clamps |
| |Hoses with adapters |
| |6 ml vials with non-septa caps |
| |Ice bath