Insecticide Resistance Action Committee www.irac-online.org

IRAC Susceptibility Test Methods Series

Method No: 008 Version: 3 (June 2009) ____________________________________________________________

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Details: Method: Status: Species: Species Stage No 008 (Formally Method No.8) Approved Bemisia tabaci Adult

Product Class:

amitraz
Photograph Courtesy of: Central Science Laboratory, Harpenden Archives, British Crown

Comments: None

Description: Materials: Clean plastic cups with push-on plastic lids, plasticine, nylon muslin with very small mesh (approximately 10.000 apertures cm-2) sharp knife or scalpel, scissors, glue, nail or other similar metal rod, untreated leaves. Method: (a) Turn a plastic lid upside down and cut a circular hole (approximately 4 cm diameter) with a scalpel. (b) Cut a roughly circular piece if muslin (approximately 5.5 cm diameter) and glue to the inside of the prepared plastic lid. (c) Heat the nail (or metal rod) and pierce a hole in the bottom of the plastic cup. (d) Select uncontaminated cotton leaves and with scissors cut the petiole to a length of approximately 4 cm. Cut the lamina to give an approximately square area (2 x 2 cm). Prepare a minimum of four replicates per treatment. (e) Prepare test liquids. The use of a wetter is not recommended. Agitate test liquids and then dip prepared leaves for 5 s holding the leaf by the petiole. (f) Leave the leaves to dry in the open air (approximately 5 min). (g) Pass the petiole of a test leaf through the hole in the plastic cup until it protrudes by approximately 1 cm. (h) Fix the petiole in position by pressing a small ball of plasticine around the petiole where it enters the cut hole.

For further information please contact the IRAC International Coordinator: Alan Porter: aporter@intraspin.com www.irac-online.org

Insecticide Resistance Action Committee www.irac-online.org

IRAC Susceptibility Test Methods Series

Method No: 008