Volumetric flask

solutions A stock solution of luteolin (0.06 mg/ml) obtained from Carl Roth GmbH & Co. was prepared by dissolving 1.5 mg luteolin in MeOH and making up the volume to 25 ml with MeOH. 1, 2, 3 and 4 ml of the stock solution were transferred to 10 ml volumetric flasks and filled to the mark with MeOH to obtain standard solutions containing 6, 12,18, 24 μg/ml, respectively. 2.4. Samples preparation Two g of pulverized raw material in 50 ml of MeOH was heated under reflux for 30 min. After filtration the procedure

similar to the one used last week. 4. Collect about 5mL of saliva in a small (~25mL) beaker. Transfer it a centrifuge tube and spin it for about five minutes. Pipet a known amount (~200 – 500 L) of the clear supernatant liquid into a 10mL volumetric flask. Fill the flask with stock iron nitrate solution. 5. Measure the solution’s absorbance at the same wavelength. 6. Use the calibration curve from the first data set to calculate the concentration of Fe(SCN)2+ in the saliva sample, and correct for the

Control website (http://www.cdc.gov/ od/ohs/biosfty/bmbl5/bmbl5toc.htm). To begin using the Sputum Digestion Solution, pour the contents of the Phosphate Buffer Powder packet into a 500 mL volumetric flask or autoclavable bottle with a 500 mL gradation. Fill with water to the 500 mL line, and mix well. If a flask was used, transfer the contents to an autoclavable bottle. Autoclave the buffer. Prepare the F.A.S.T. Sputum Digestion Solution by first loosening (but not removing) the cap of the plastic

growth. The pH value was maintained at 5. Fifty millilitres of sample were taken into 100-ml Erlenmeyer flasks and autoclaved at 121.0 °C for 30 min. The sterile medium was cooled to ambient temperature and inoculated with 2.0% (v/v) spore suspension of A. niger strain and incubated at 30 °C for periods of 24, 48, 72, 96 and 120 h and agitated at 150 rpm. For each incubation period, a separate set of flasks with medium were used. For reproducibility of results, all fermentations were carried out in triplicate

of approximately 80° brix and therefore, their dissolution is a critical point. The syrups are mixed manually and thoroughly to insure their homogeneity and the achievement of a representative sampling. Five grams of syrup are then added to a volumetric flask and made up with 35 °C purified water. The solutions were then sonnicate. Depending on the sample, these stock solutions were diluted from 20 to 50 times before analysis. 56 CHAPTER 4 : MATERIAL AND METHODS I2 P Glc M2 GA I-P + M3 I3

dévitrifier Devonian n. (géol.), Dévonien m. devour (to) v. t. (zoo.), dévorer dew n. (phys.), rosée f., ~ point, point de rosée, ~ point meter (instr.), appareil de mesure du point de rosée dewar n. (instr., thermo.), dewar m., bouteille f. thermos, ~ flask, vase Dewar, dewar, ~ vessel, vase Dewar, dewar dewclaw n. (zoo.), ergot m. dewet (to) v. t., ne plus mouiller dextran n. (bio.), dextrane m. dextrin n. (chim.), dextrine f. dextroglucose = dextrose dextrogyrate = dextro(-)rotary dextrorota(to)ry adj

dévitrifier Devonian n. (géol.), Dévonien m. devour (to) v. t. (zoo.), dévorer dew n. (phys.), rosée f., ~ point, point de rosée, ~ point meter (instr.), appareil de mesure du point de rosée dewar n. (instr., thermo.), dewar m., bouteille f. thermos, ~ flask, vase Dewar, dewar, ~ vessel, vase Dewar, dewar dewclaw n. (zoo.), ergot m. dewet (to) v. t., ne plus mouiller dextran n. (bio.), dextrane m. dextrin n. (chim.), dextrine f. dextroglucose = dextrose dextrogyrate = dextro(-)rotary dextrorota(to)ry adj